head of the group:
professor:
prof. RNDr. Milan Kodíček, CSc.
associate professor:
doc. Ing. Mgr. Štěpánka Kučková, Ph.D.
assistant professors:
Ph.D. Students:
Ing. Iva Pitelková
Ing. Lucie Maršálová
Ing. Lucie Coufalová
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Also available at Zenodo
Department of biochemistry and microbiology focus on basic research in areas of retroviral molecular biology, proteomics, plant physiology and molecular genetics, enzymology, environmental microbiology, food microbiology and bioanalytical methods. These activities create a platform for applied research aimed at developing modern therapeutic approaches, bioremediation of inorganic and organic pollutants, monitoring food safety and quality (the department also operates accredited Testing Laboratory of the Institute of Biochemistry and Microbiology) or plant-pathogen interaction. Research in our department is in many cases interdisciplinary and, in addition to close professional cooperation between the individual laboratories of the department, it would be unthinkable without cooperation with a number of national and foreign groups within the framework of joint research programs and projects. Currently, we are working on projects supported by the Czech Science Foundation, Technology Agency of the Czech Republic, Ministry of Agriculture of the Czech Republic, Ministry of Industry and Trade of the Czech Republic and the Ministry of Education and Sports.
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Our lab group is focused on applications of the proteomics approaches using mass spectrometry as a detection endpoint. Using tools of the mass spectrometry we can study a broad spectrum of biological systems on the molecular level. Proteomics can be characterised as a field of the biochemistry that follows up complex studying of proteins in the specific time under defined conditions and is the one of the most dynamic and evolving science fields.
For the studying of proteins we use the mass spectrometric techniques: MALDI-TOF/TOF and LC-ESI-Q-TOF.
Examples of Research Topics
- Identification of proteins involved in crop stress reaction
- Studying of membrane complexes associated with signal paths of plants
- Proteomics aspects of mineralized heart valves
- Protein analysis of artworks and historical mortars
- Proteomic analysis of alveolar bones in connection with oral surgery
- Proteomic analyses of bioliquids of patients with carcinomas and neurodenerative diseases
- Identification of zooplankton
- Distinguishing of animal species origin of parchments and hairs
- Proteomics analysis of retroviruses
- Identification of pathogen microorganisms
- Development of techniques for protein quantification by mass spectrometry (methods iTRAQ, dimethyl labelling, TOP3 etc)
Methods
- Peptide mass fingerprinting → protein identification
- Surface mapping → study of protein conformation
- Biotyping → identification of microorganism
- Protein quantification (iTRAQ, dimethyl labelling, SIM, TOP3, MeanInt)
- Cross-linking and pull-down of membrane proteins
head of the group:
professor:
prof. RNDr. Milan Kodíček, CSc.
associate professor:
doc. Ing. Mgr. Štěpánka Kučková, Ph.D.
assistant professors:
Ph.D. Students:
Ing. Iva Pitelková
Ing. Lucie Maršálová
Ing. Lucie Coufalová
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