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Technická 5
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Department of Biochemistry and Microbiology is dedicated to higher learning and research in the fields of biology, biochemistry, microbiology and molecular biology. We organise courses in core biochemical disciplines for the Faculty of Food and Biochemical Technology in Bachelor and Master programme. Other specialized courses are offered for Master and postgraduate students. Student in all stages of higher learning are actively participating in our research projects.

UCT (originál)

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Main spectrum projections of "pseudo-Cronobacter" strains from the article B. Svobodová, J. Vlach, P. Junková, L. Karamonová, M. Blažková & L. Fukal: Novel Method for Reliable Identification of Siccibacter and Franconibacter Strains: from “Pseudo-Cronobacter” to New Enterobacteriaceae Genera. Applied and Environmental Microbiology 83(13) e00234-17. DOI: 10.1128/AEM.00234-17.

EB29_Franconibacter_helveticus_MspExport.btmsp
EB31_Franconibacter_helveticus_MspExport.btmsp
EB32_Franconibacter_helveticus_MspExport.btmsp
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EB45_Siccibacter_turicensis_MspExport.btmsp

Also available at Zenodo

DOI
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Department of biochemistry and microbiology focus on basic research in areas of retroviral molecular biology, proteomics, plant physiology and molecular genetics, enzymology, environmental microbiology, food microbiology and bioanalytical methods. These activities create a platform for applied research aimed at developing modern therapeutic approaches, bioremediation of inorganic and organic pollutants, monitoring food safety and quality (the department also operates accredited Testing Laboratory of the Institute of Biochemistry and Microbiology) or plant-pathogen interaction. Research in our department is in many cases interdisciplinary and, in addition to close professional cooperation between the individual laboratories of the department, it would be unthinkable without cooperation with a number of national and foreign groups within the framework of joint research programs and projects. Currently, we are working on projects supported by the Czech Science Foundation, Technology Agency of the Czech Republic, Ministry of Agriculture of the Czech Republic, Ministry of Industry and Trade of the Czech Republic and the Ministry of Education and Sports.

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Department of Biochemistry and Microbiology offers bachelor courses in core disciplines (biochemistry, microbiology, biology and others). We also participate on courses for the novel bachelor program focused on forensic sciences. Biochemistry courses are offered to students of all faculties of our university. Our department organizes master programmes General and Applied Biochemistry, Microbiology and Clinical Biochemistry.

Advanced Biochemistry, Applied Enzymology, Environmental Microbiology, Food microbiology and Genetic engineering are delivered in English for foreign students. 

The department is accredited for doctoral education of biochemists and microbiologists.

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Content of english website is limited. We are working on translation.

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DATA


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    [nazev] => Laboratory of Molecular Biology and Virology
    [seo_title] => Photoactive molecules
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Home   Publications

Photobiology of materials based on Mo6I8 clusters and porphyrin frameworks

The molecular clusters based on Mo6I8 core with variable outer ligands and the porphyrin frameworks share the ability to catalyze the production of highly reactive singlet oxygen upon illumination with light or irradiation with X-rays. They serve for the preparation of soluble molecules, nanolayers, or nanoparticles that could kill cancer cells or pathogenic bacteria upon illumination or irradiation. In collaboration with Dr. Kamilem Langem a Dr. Kaplanem Kirakci from the Institute of Inorganic Chemistry, Czech Academy of Sciences, our group test the biological properties of such novel materials. We culture normal and cancer cells of human origin in our tissue culture facility and illuminate them with special light sources or with X-rays. Our hypoxic chamber allows us to work under low physiologically relevant level of oxygen. We can determine the cellular uptake and localization of cluster compounds or nanoparticles with confocal microscope or with structure illumination super-resolution microscope (SIM). The nanoparticles are also specifically targeted to cancer cells using molecular recognition. The promising materials are further tested on mouse models in collaboration with Dr. Milanem Reinišem from Institute of Molecular Genetics, Czech Academy of Sciences. The antibacterial properties of compounds, layers and particles are determined using our microbiology facility and model strains of bacteria relevant in clinics. The bacteria are cultured in the form of biofilm, a naturally occurring multicellular structure with enhanced physical and chemical resistance.

fig1
Principle of photosensitizing effect of a molybdenum cluster.

fig2
Microscopic colocalization of molybdenum cluster (red) and lysosomes (green) in cancer cell.

fig3
Microscopic colocalization of molybdenum cluster (red) and Enterococcus faecalis bacteria.

fig4
Antibacterial effects of the materials are confirmed by cultivation methods.

Funding

  • Biomaterials based on octahedral molybdenum clusters as singlet oxygen radiosensitizers, GA18-05076S, Czech Science Fundation

Publications

  1. Kirakci K., Nguyen T.K.N., Grasset F., Uchikoshi T., Zelenka J., Kubát P., Ruml T., Lang K.: Electrophoretically Deposited Layers of Octahedral Molybdenum Cluster Complexes: A Promising Coating for Mitigation of Pathogenic Bacterial Biofilms under Blue Light. ACS Appl Mater Interfaces, 2020. IF 8,758
  2. Kirakci K., Demel J., Hynek J., Zelenka J., Rumlová M., Ruml T., Lang K..: Phosphinate Apical Ligands: A Route to a Water-Stable Octahedral Molybdenum Cluster Complex. Inorg Chem 58:16546–16552, 2019. IF 4,850
  3. Kirakci K., Zelenka J., Rumlová M., Cvačka J., Ruml T., Lang K.: Cationic octahedral molybdenum cluster complexes functionalized with mitochondria-targeting ligands: photodynamic anticancer and antibacterial activities. Biomater Sci 7:1386-1392, 2019. IF 5,831
  4. Kirakci K., Zelenka J., Rumlová M., Martinčík J., Nikl M., Ruml T., Lang K.: Octahedral molybdenum clusters as radiosensitizers for X-ray induced photodynamic therapy. J Mater Chem B 6:4301-4307, 2018. IF 5,047
  5. Hynek J., Zelenka J., Rathouský J., Kubát P., Ruml T., Demel J., Lang K.: Designing Porphyrinic Covalent Organic Frameworks for the Photodynamic Inactivation of Bacteria. ACS Appl Mater Interfaces 10:8527-8535, 2018. IF 8,456
  6. Buzek D., Zelenka J., Ulbrich P., Ruml T., Krizova I., Lang J., Kubat P., Demel J., Kirakci K., Lang K.: Nanoscaled porphyrinic metal-organic frameworks: photosensitizer delivery systems for photodynamic therapy. J Mater Chem B 5:1815-1821, 2017. IF 5,047
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