Home Projects Accredited testing laboratory of the DBM FFBT UCT Prague
Over the last two centuries, microbiology and related biological sciences have undergone tremendous development, and current knowledge only confirms the statements of the most important microbiologist of the 19th century, Louis Pasteur. Our microbiology laboratory interest falls into several areas. The main one is food microbiology, which is also due to the histological aspects and the fact that the Department of Biochemistry and Microbiology falls under the Faculty of Food and Biochemical Technology. However, the scope of the laboratory has expanded considerably in the last decades. Since 2004, we run the accredited Testing Laboratory for Food Microbiology and Genetically Modified Organisms (GMO). Further, we are currently addressing several topics in the field of applied microbiology, such as antibiotic resistance and microbial interactions with various (nano)material.
Areas of focus of our working group:
- Food microbiology
- Applied Microbiology
- GMO
Food pathogens we work with:
- Salmonella sp., Escherichia coli, Cronobacter sp., Campylobacter jejuni / coli, Staphylococcus aureus, Listeria monocytogenes
What we are interested in:
- microbial identification by conventional and modern, rapid methods
- genotyping
- antibiotic resistance
- expression of virulence factors and other selected genes
- enterotoxin production in S. aureus
- biofilm formation and biofilm resistance to antimicrobial agents
- thermoresistance
Methods we use:
- culture and identification methods of conventional food microbiology, including ISO methods
- MALDI-TOF MS for microorganisms identification, SNP detection, and beta-lactamase activities detection
- DNA and RNA isolation
- detection and identification of food relevant microorganisms by PCR
- detection and quantification of genes by end-point PCR and qPCR
- transcription of selected bacterial genes by qPCR
- sequencing (Sanger sequencing, nanopore sequencing)
- genotyping (PFGE, AFLP, RAPD, ERIC, MLST, mP-BiT)
- microscopy (CLSM, SEM)
- bioluminescence analysis of signalling molecule activities by quorum sensing
