The Microbiology Division focuses on various microbiology topics surrounding metabolism, oxidative stress, and research for new antimicrobial compounds. Redox laboratory is dedicated to exploring the intricate mechanisms by which light, oxidative stress, and other environmental factors impact microorganisms at the cellular and molecular levels. We work with either common bacterial species or yeasts.
Photodynamic inactivation of bacteria (PDI)
We investigate the fascinating world of photoinactivation, a process based on the same principle as photodynamic therapy by which bacteria are rendered nonviable by reactive oxygen species (ROS), generated by photosensitizers exposed to specific wavelengths of light. By delving into the intricacies of this phenomenon, we aim to develop novel methods for disinfection and sterilization, potentially revolutionizing fields such as healthcare, food safety, and water treatment.
We test novel antibacterial compounds, such as molybdenum clusters, and other photosenzitizers for PDI such as well-researched methylene blue or nanorobots. We evaluate toxic effects against planktonic forms of bacteria or more resistant, commonly occurring biofilm forms.
Oxidative stress in microorganisms
Additionally, the division places a significant emphasis on oxidative stress in bacteria and yeasts. Oxidative stress occurs when cells are subjected to an imbalance between reactive oxygen species (ROS) production and their detoxification mechanisms. We seek to unravel how microorganisms and yeasts respond to oxidative stress and adapt to their environments. This research is vital in understanding the virulence and survival strategies of pathogenic microorganisms, as well as the role of yeasts in various biotechnological processes.
We use various techniques of cultivation, antioxidant capacity assays, ROS generation detection using different fluorescent probes, optical microscopy, confocal fluorescent microscopy, and more.
